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Lepidopteran Insect Cell Line Isolation from Insect Tissue
1INSell Consulting, 247 Lynch Rd, Newcastle, ME, 04553-3929, USA. ikehiker@gmail.com.
Methods in Molecular Biology (Clifton, N.J.)
|January 29, 2016
Abstract:
This chapter describes procedures for initiating new cell lines from lepidopteran larval tissues and embryos. The internal morphology is described along with methods for treating excised tissues and the primary cultures. Advice on culture medium and the tissues that will provide the best chance for new cell lines is discussed.
