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Updated: Mar 26, 2026

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In Situ Hybridization of Breast Cancer Markers

Li Min1, Chengchao Shou2

  • 1Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, 52# Fu-Cheng Road, Haidian District, Beijing, 100142, People's Republic of China.

Methods in Molecular Biology (Clifton, N.J.)
|January 29, 2016
PubMed

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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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Keywords:
Breast cancerCISHFISHHER2In situ hybridization