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Updated: Mar 26, 2026

Using the Dot Assay to Analyze Migration of Cell Sheets
Published on: December 5, 2017
A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration
Vincent M Alford1, Eric Roth1, Qian Zhang1
1Division of Cancer Prevention, Department of Medicine, Stony Brook University, 101 Nicolls Road, Stony Brook, NY, 11794, USA.
Abstract:
Cell migration is a critical determinant of cancer invasion and metastasis. Drugs targeting cancer cell migration have been hindered due to the lack of effective assays for monitoring cancer cell migration. Here we describe a novel method to microscopically monitor cell migration in a quantitative fashion. This assay can be used to study genes involved in cancer cell migration, as well as screening anticancer drugs that target this cellular process.
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