Related Experiment Video
Updated: Mar 26, 2026

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
Published on: May 1, 2019
Challenges of glycosylation analysis and control: an integrated approach to producing optimal and consistent
Peiqing Zhang1, Susanto Woen2, Tianhua Wang2
1Bioprocessing Technology Institute, 20 Biopolis Way, #06-01 Centros, Singapore; National University of Singapore, 21 Lower Kent Ridge Road, Singapore.
Abstract:
Glycosylation of therapeutic proteins has a profound impact on their safety and efficacy. Many factors shape the glycosylation of biotherapeutics, ranging from expression systems and cell culture processes to downstream purification strategies. Various analytical technologies have been developed to address questions concerning different aspects of glycosylation. Informatics tools are also crucial for a systematic understanding of the glycosylation processes. Hence, an integrated approach is required to harness glycosylation for the production of optimal and consistent glycoprotein-based therapeutic drugs. Here, we review the latest developments and challenges in glycosylation analysis and control in the context of bioprocessing monoclonal antibodies.
Related Concept Videos
Protein Glycosylation
Glycosylation occurs in...
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Proteoglycans

