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Updated: Mar 26, 2026
Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies
Published on: April 11, 2012
Histopathological and Biochemical Effects of Ecballium elaterium on Sepsis-Induced Lung Injury
Melike Demir1, Mahsuk Taylan1, Halide Kaya1
1a Department of Chest Disease , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
Purpose:
The aim of this study was to investigate the role of Ecballium elaterium (EE) on sepsis-induced lung injury.
Materials And Methods:
A total of 30 male rats were divided into three groups as follows: control, sepsis, and treatment groups (sepsis + EE) with each group containing 10 rats. A rat model of sepsis induced by cecal ligation and puncture (CLP) was used. In the treatment group, rats were injected intraperitoneally with 2.5 mg/kg EE after CLP. Interleukin-6 (IL-6), tumor necrosis factor (TNF)-α, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values after a 24-hr period were measured via cardiac puncture. Animals were harvested after the procedure and biochemical analysis was done and histopathological changes of the tissue sections of lungs were examined thereafter.
Results:
A statistically significant decrease was observed in the IL-6 (p < .05), TNF-α (p < .05), and TOS (p < .01) levels in the sera of the treatment group compared to those of the sepsis group. Following the treatment, the TOS (p = .01) and OSI (p < .05) levels in the lung tissue of rats indicated a statistically significant decrease compared to those of the sepsis group. The histopathological follow-up undertaken after the administration of the EE treatment to septic rats showed significantly lower values of alveolar wall thickness (p < .001), interstitial edema (p = .018), and neutrophil infiltration (p = .047).
Conclusion:
EE treatment may have beneficial effects on sepsis-induced lung injury, and therefore has potential for clinical use.
