Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrogel oxygen reservoirs increase functional integration of neural stem cell grafts by meeting metabolic demands.

Nature communications·2023
Same author

The effect of left ventricular longitudinal strain on left atrial function and ventricular filling in hypertension.

Clinical radiology·2022
Same author

Electroactive Amphiphiles for Addressable Supramolecular Nanostructures.

ChemNanoMat : chemistry of nanomaterials for energy, biology and more·2019
Same author

Complex three-dimensional self-assembly in proxies for atmospheric aerosols.

Nature communications·2017
Same author

The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III).

Biochemical and biophysical research communications·2017
Same author

Lactobacillus fermentum 3872 as a potential tool for combatting Campylobacter jejuni infections.

Virulence·2017

Related Experiment Video

Updated: Mar 26, 2026

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
10:23

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Published on: December 13, 2016

10.5K

Ultra-fast stem cell labelling using cationised magnetoferritin.

S Correia Carreira1, J P K Armstrong2, A M Seddon1

  • 1Bristol Centre for Functional Nanomaterials, University of Bristol, Tyndall Avenue, Bristol, BS8 1FD, UK. S.Carreira@bristol.ac.uk and H.H. Wills Physics Laboratory, University of Bristol, Tyndall Avenue, Bristol, BS8 1TL, UK. W.Schwarzacher@bristol.ac.uk.

Nanoscale
|January 30, 2016
PubMed
Summary
This summary is machine-generated.

Magnetoferritin nanoparticles rapidly magnetize human mesenchymal stem cells (hMSCs) in just one minute. This novel method offers a biocompatible and efficient platform for cell-based therapies, enhancing magnetic resonance imaging contrast.

More Related Videos

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
05:05

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

Published on: November 4, 2011

21.9K
Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
09:06

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Published on: March 31, 2008

10.3K

Related Experiment Videos

Last Updated: Mar 26, 2026

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
10:23

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Published on: December 13, 2016

10.5K
Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
05:05

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

Published on: November 4, 2011

21.9K
Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
09:06

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Published on: March 31, 2008

10.3K

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Regenerative Medicine

Background:

  • Superparamagnetic iron oxide nanoparticles (SPIONs) are crucial for cell imaging and manipulation.
  • Current SPION labeling methods require long incubation times or surface functionalization, potentially harming cell function.

Purpose of the Study:

  • To develop a rapid and biocompatible method for magnetic cell labeling using functionalized magnetoferritin.
  • To evaluate the efficiency and safety of cationized magnetoferritin for labeling human mesenchymal stem cells (hMSCs).

Main Methods:

  • Chemical cationization of magnetoferritin to create a membrane-active nanoparticle.
  • Incubation of hMSCs with cationized magnetoferritin for short durations (as little as one minute).
  • Assessment of cell magnetization, MRI contrast enhancement, membrane integrity, proliferation, and differentiation capacity.

Main Results:

  • Cationized magnetoferritin enabled rapid (1-minute) magnetization of hMSCs.
  • Magnetization was stable for several weeks and provided significant T2* MRI contrast enhancement.
  • No adverse effects on hMSC membrane integrity, proliferation, or multi-lineage differentiation were observed.

Conclusions:

  • Cationized magnetoferritin offers a fast, efficient, and non-cytotoxic nanoparticle system for magnetic cell labeling.
  • This platform is suitable for cell-based therapies in regenerative medicine, improving MRI contrast.
  • The rapid labeling and biocompatibility make magnetoferritin a versatile tool for biotechnological applications.