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Activation of the cAMP/protein kinase A signalling pathway by coronin 1 is regulated by cyclin-dependent kinase 5

Xiaolong Liu1, Somdeb BoseDasgupta1, Rajesh Jayachandran1

  • 1Biozentrum, University of Basel, Switzerland.

FEBS Letters
|January 30, 2016
PubMed

Insights

Cyclin-dependent kinase 5 (CDK5) activity promotes the coronin 1-dependent cAMP/PKA pathway by phosphorylating coronin 1, which facilitates its association with Gαs. This reveals a key mechanism for regulating neuronal signaling.

Area of Science:

  • Molecular and Cellular Neuroscience
  • Signal Transduction Pathways
  • Protein Phosphorylation

Background:

  • Coronins are conserved eukaryotic proteins regulating diverse cellular activities.
  • Coronin 1 is known to modulate the cAMP/PKA pathway in neurons via interaction with Gαs.
  • The precise mechanism governing coronin 1-Gαs interaction remained undefined.

Purpose of the Study:

  • To elucidate the molecular mechanism regulating coronin 1-Gαs interaction.
  • To identify the role of specific post-translational modifications in this interaction.
  • To understand the upstream regulators of coronin 1's function in the cAMP/PKA pathway.

Main Methods:

  • Investigated the phosphorylation status of coronin 1.
  • Utilized biochemical assays to assess protein-protein interactions.
  • Measured cAMP production in response to signaling stimuli.

Main Results:

  • Demonstrated that phosphorylation of coronin 1 at Thr(418/424) by CDK5 is critical for Gαs binding.
  • Showed that this phosphorylation event is stimulus-dependent.
  • Confirmed that CDK5 activity directly influences coronin 1-mediated cAMP production.

Conclusions:

  • CDK5 activity is essential for promoting the coronin 1-dependent cAMP/PKA signaling pathway.
  • Phosphorylation of coronin 1 by CDK5 serves as a key regulatory switch for Gαs interaction.
  • This finding provides a novel molecular insight into neuronal signaling regulation.
Keywords:
CDK5Coronin 1G protein alpha SPhosphorylationcAMP/PKA signalling

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