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Manipulating the inter pillar gap in pillar array ultra-thin layer planar chromatography platforms.

Nichole A Crane1, Nickolay V Lavrik2, Michael J Sepaniak1

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|January 30, 2016
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Summary
This summary is machine-generated.

Reducing the gap between pillars in micro- and nanofabricated separation platforms enhances chromatographic efficiency. Narrowing pillar gaps and diameters simultaneously offers the best approach for improved separation performance.

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Area of Science:

  • Microfluidics and Nanofabrication
  • Chromatographic Separation Science

Background:

  • Deterministic micro- and nanofabrication offers precise control over morphological parameters in separation platforms.
  • Traditional packed-bed systems lack the fine-tuned control achievable with lithographically-prepared pillar arrays.

Purpose of the Study:

  • To investigate the impact of reducing inter-pillar gaps on capillary action-driven flow and band dispersion in pillar array separation platforms.
  • To optimize chromatographic efficiency by manipulating morphological parameters.

Main Methods:

  • Utilized atomic layer deposition to reduce inter-pillar gaps in photo-lithographically defined pillar arrays.
  • Modeled plate height based on predicted and observed flow rates in gap-adjusted arrays.
  • Correlated flow rate reductions with separation efficiency.

Main Results:

  • A reduction in flow rate was observed with smaller inter-pillar gaps.
  • This reduced flow rate negatively impacted efficiency in modeled scenarios.
  • Observed flow rate changes correlated with actual chromatographic separations.

Conclusions:

  • Simultaneously reducing both inter-pillar gap and pillar diameter is the most effective strategy for enhancing chromatographic efficiency.
  • Morphological parameter control is key to optimizing separation performance in microfabricated devices.