Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Construction of a 3D printed, human gingival MSC seeded alveolar bone implant.

Journal of applied biomaterials & functional materials·2026
Same author

Modeling a hypoxia-integrated glioblastoma microenvironment to mimic tumor heterogeneity and chemoresistance.

Biomaterials science·2026
Same author

Biomimetic mineralization of electrospun elastin-like recombinamer nanofibers.

Scientific reports·2026
Same author

Development of a tissue-engineered oral mucosal barrier model using poly (L-lactic acid) electrospun nanofibers.

Scientific reports·2026
Same author

Nerve tissue model on a micropatterned surface: Axon guidance and neural regeneration.

Journal of materials science. Materials in medicine·2025
Same author

Establishment of an In Vitro Neurovascular Unit Model With Blood and Brain Components and Investigation of Its Blood-Brain Barrier.

Journal of biomedical materials research. Part A·2025

Related Experiment Video

Updated: Mar 26, 2026

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

13.0K

A collagen-based corneal stroma substitute with micro-designed architecture.

Cemile Kilic1, Alessandra Girotti2, J Carlos Rodriguez-Cabello2

  • 1Department of Biological Sciences, METU, Ankara, Turkey and Department of Biotechnology, METU, Ankara, Turkey and BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, METU, Ankara, Turkey.

Biomaterials Science
|February 2, 2016
PubMed
Summary

Researchers developed a 3D corneal stroma substitute using patterned collagen films. These biomaterials show high transparency and support human corneal keratocyte (HK) growth, indicating potential for corneal regeneration therapies.

More Related Videos

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
05:20

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Published on: February 6, 2020

8.5K
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
07:35

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

9.4K

Related Experiment Videos

Last Updated: Mar 26, 2026

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

13.0K
Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
05:20

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Published on: February 6, 2020

8.5K
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
07:35

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

9.4K

Area of Science:

  • Biomaterials Science
  • Ophthalmology
  • Tissue Engineering

Background:

  • Corneal stromal defects require effective tissue substitutes.
  • Developing biocompatible materials that mimic native corneal structure is crucial.

Purpose of the Study:

  • To engineer a 3D corneal stroma substitute using patterned collagen and collagen-elastin like recombinamer (Col:ELR) blend films.
  • To evaluate the transparency, cell attachment, proliferation, and alignment of human corneal keratocytes (HK) on these constructs.

Main Methods:

  • Fabrication of 3D constructs by stacking 4 micro-patterned Col or Col:ELR blend films.
  • Assessment of film transparency (uncrosslinked and dehydrothermally treated).
  • In vitro evaluation of HK attachment, proliferation, and alignment on patterned and unpatterned films and constructs.

Main Results:

  • High transparency was observed in uncrosslinked and dehydrothermally treated Col:ELR films.
  • HK attached and proliferated well on single Col and Col:ELR films, with higher proliferation on Col in multilayer constructs.
  • Cells aligned along the micro-patterns of the films, demonstrating contact guidance.

Conclusions:

  • Col and Col:ELR films and their 3D constructs show significant potential as corneal stroma substitutes.
  • The patterned constructs promote cellular alignment, mimicking native corneal tissue.
  • The high transparency and biocompatibility support their use in regenerative medicine for corneal repair.