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Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
A biosensor based on gold nanoparticles, dihexadecylphosphate, and tyrosinase for the determination of catechol in
Fernando Campanhã Vicentini1, Lívia L C Garcia2, Luiz C S Figueiredo-Filho3
1Department of Chemistry, Federal University of São Carlos, Rod. Washington Luís km 235, P.O. Box 676, 13560-970 São Carlos, SP, Brazil; Center of Nature Sciences, Federal University of São Carlos, Rod. Lauri Simões de Barros km 12, 18290-000 Buri, SP, Brazil.
Abstract:
In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and cyclic voltammetry in the presence of catechol. The determination of catechol was carried out by amperometry and presented a linear concentration range from 2.5×10(-6) to 9.5×10(-5)molL(-1) with a detection limit of 1.7×10(-7)molL(-1). The developed biosensor showed good repeatability and stability. Moreover, this novel amperometric method was successfully applied in the determination of catechol in natural water samples. The results were in agreement with a 95% confidence level for those obtained using the official spectrophotometric method.
