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Published on: October 10, 2017
TruSPAdes: barcode assembly of TruSeq synthetic long reads
Anton Bankevich1, Pavel A Pevzner1,2
1Center for Algorithmic Biotechnology, Institute for Translational Biomedicine, Saint Petersburg State University, Saint Petersburg, Russia.
Abstract:
The recently introduced TruSeq synthetic long read (TSLR) technology generates long and accurate virtual reads from an assembly of barcoded pools of short reads. The TSLR method provides an attractive alternative to existing sequencing platforms that generate long but inaccurate reads. We describe the truSPAdes algorithm (http://bioinf.spbau.ru/spades) for TSLR assembly and show that it results in a dramatic improvement in the quality of metagenomics assemblies.
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