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Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Published on: November 16, 2018
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Transcriptome modifications in human gingival fibroblasts exposed to 2-hydroxyethyl methacrylate.
Chiara Di Nisio1, Marco D'Aurora2, Viviana di Giacomo1
1Department of Pharmacy, "G.d'Annunzio" University, Via Dei Vestini 31, 66100 Chieti-Pescara, Italy.
Gene
|February 2, 2016
Summary
Low concentrations of 2-Hydroxyethyl methacrylate (HEMA) impact human gingival fibroblast (HGF) gene expression, causing damage and impairing proliferation. Early HEMA exposure triggers repair, while prolonged exposure induces apoptosis.
Area of Science:
- Biomaterials Science
- Molecular Biology
- Dental Materials
Background:
- 2-Hydroxyethyl methacrylate (HEMA) is a common dental restorative material.
- HEMA has demonstrated toxic effects on various cell types, including human gingival fibroblasts (HGFs) and odontoblasts.
- Understanding HEMA's cellular impact is crucial for dental material safety.
Purpose of the Study:
- To investigate the differential transcriptome modulation induced by low HEMA concentrations in cultured HGFs.
- To analyze the gene expression changes at early (24 h) and later (96 h) time points.
- To elucidate the cellular response mechanisms to HEMA exposure.
Main Methods:
- Cultured HGFs were exposed to 3 mmol/l HEMA for 24 or 96 hours.
- Whole genome microarray analysis was performed on extracted RNA.
- Differential gene expression and functional pathway analysis were conducted.
Main Results:
- A total of 310 transcripts were differentially expressed between HEMA-treated and control groups at 24 and 96 hours.
- Affected genes were primarily involved in cellular survival, death, and inflammatory responses.
- HEMA exposure led to overall cellular damage and impaired proliferation at both time points.
Conclusions:
- HEMA at low concentrations significantly alters HGF transcriptome, impacting cellular functions.
- Short-term HEMA exposure (24 h) may initiate compensatory repair mechanisms.
- Long-term HEMA exposure (96 h) results in apoptosis, indicating chronic damage and cell death.
