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Differentiation of a resistant clone of mouse myeloid leukemia cells with dimethyl sulfoxide and ascitic fluid
Abstract:
Mouse myeloid leukemia line cells, M1, could be induced to differentiate in vitro into macrophages and granulocytes with ascitic fluid of animals bearing various tumors. M1 cells could not be induced to differentiate with dimethyl sulfoxide alone. During the culture of M1 cells, spontaneously appearing cells resistant to factors stimulating differentiation (D-factor) in ascitic fluid were isolated. These resistant cells were more refractile to the toxic action of dimethyl sulfoxide than sensitive cells and grew in culture medium with 1% dimethyl sulfoxide. Although the resistant cells were not induced to differentiate with dimethyl sulfoxide alone, they were sensitized with the aid of dimethyl sulfoxide to undergo differentiation with the D-factor in ascitic fluid.
Insights
Mouse myeloid leukemia (M1) cells differentiate into macrophages and granulocytes using tumor ascitic fluid. Resistant M1 cells, identified and isolated, showed altered responses to dimethyl sulfoxide and differentiation factors.
Area of Science:
- Cell Biology
- Cancer Research
- Hematopoiesis
Background:
- Mouse myeloid leukemia (M1) cell line serves as a model for studying myeloid differentiation.
- Tumor-associated ascites fluid contains factors that can induce M1 cell differentiation.
- Dimethyl sulfoxide (DMSO) is a known inducer of M1 cell differentiation, but its mechanism and limitations are areas of study.
Purpose of the Study:
- To investigate the characteristics of M1 cells resistant to differentiation-inducing factors.
- To explore the interaction between dimethyl sulfoxide and differentiation factors in M1 cells.
- To understand the mechanisms underlying resistance and sensitization to differentiation stimuli.
Main Methods:
- Culture of M1 cells with tumor ascites fluid and/or dimethyl sulfoxide.
- Isolation and characterization of M1 cell variants resistant to differentiation.
- Assessment of cell refractility and growth in the presence of dimethyl sulfoxide.
- Evaluation of differentiation induction in resistant cells under varying conditions.
Main Results:
- M1 cells differentiated into macrophages and granulocytes when treated with tumor ascites fluid.
- Spontaneously arising M1 cell variants resistant to differentiation factors were isolated.
- These resistant cells exhibited increased refractility to dimethyl sulfoxide toxicity and could grow in 1% DMSO.
- While resistant cells did not differentiate with DMSO alone, DMSO pre-treatment sensitized them to differentiation by ascites fluid factors.
Conclusions:
- Tumor ascites fluid contains differentiation-inducing factors (D-factor) for M1 cells.
- M1 cell differentiation resistance can arise, with altered sensitivity to DMSO and D-factor.
- Dimethyl sulfoxide can modulate M1 cell differentiation sensitivity, potentially overcoming resistance in combination with D-factor.