Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

1.6K
Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
1.6K
Introduction to Metabolism01:30

Introduction to Metabolism

3.5K
Metabolism encompasses all biochemical reactions in a living organism, facilitating both the breakdown and synthesis of biomolecules. These metabolic processes are categorized into catabolic and anabolic pathways, which operate in a coordinated manner to ensure energy balance and cellular function.Catabolic Pathways and Energy ReleaseCatabolic pathways involve the breakdown of complex macromolecules such as carbohydrates, lipids, and proteins into smaller structures like monosaccharides, fatty...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Commensal-derived acetylcholine enhances mucosal immune education.

Nature·2026
Same author

A drug-microbiome-drug interaction impacts co-prescribed medications for Parkinson's disease.

Nature microbiology·2026
Same author

Natural Products as Signals across Microbiomes and the Environment.

Journal of natural products·2026
Same author

CAR-T-drug conjugate against solid tumor.

bioRxiv : the preprint server for biology·2026
Same author

Three-component assembly and structure-function relationships of (-)-gukulenin A.

Science (New York, N.Y.)·2025
Same author

Discovery of a Widespread Polyamine-Low-Molecular-Weight Thiol Hybrid Pathway in <i>Clostridioides difficile</i>.

ACS infectious diseases·2025

Related Experiment Video

Updated: Mar 26, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

1.4K

Secondary Metabolic Pathway-Targeted Metabolomics.

Maria I Vizcaino1,2, Jason M Crawford3,4,5,6

  • 1Department of Chemistry, Yale University, New Haven, CT, 06510, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 3, 2016
PubMed
Summary
This summary is machine-generated.

This study details methods for building molecular networks to understand microbial natural product biosynthesis. These techniques aid in discovering new natural products by analyzing metabolic pathways using mass spectrometry and network analysis.

Keywords:
Chemical signalingComparative metabolomicsHigh-resolution mass spectrometryIsotopic labelingMolecular networkingNatural product discoveryNonribosomal peptide biosynthesisSecondary metabolism

More Related Videos

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.9K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.6K

Related Experiment Videos

Last Updated: Mar 26, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

1.4K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.9K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.6K

Area of Science:

  • Microbiology
  • Biochemistry
  • Natural Products Chemistry

Background:

  • Microbial natural products are crucial for drug discovery.
  • Understanding their biosynthesis pathways is complex.
  • Orphan biosynthetic pathways present significant challenges.

Purpose of the Study:

  • To provide step-by-step methods for building secondary metabolic pathway-targeted molecular networks.
  • To enable systems-level assessment of microbial natural product biosynthesis.
  • To facilitate downstream natural product discovery efforts.

Main Methods:

  • High-resolution mass spectrometry (HRMS)-based comparative metabolomics.
  • Pathway-targeted tandem mass spectrometry (MS/MS) molecular networking.
  • Isotopic labeling for elucidating natural products from orphan pathways.
  • Utilizing chemometric platforms (e.g., Agilent Mass Profiler Professional) and online networking (GnPS).

Main Results:

  • A comprehensive workflow for metabolomics network analysis is presented.
  • Differential analysis identifies pathway-dependent molecular features.
  • Pathway-dependent networks are built using targeted MS/MS data.
  • Integration of genetic and experimental data enhances network analysis.

Conclusions:

  • The developed molecular networking approach facilitates systems biosynthesis analysis.
  • This methodology aids in assessing experimental perturbations.
  • The approach effectively guides novel natural product discoveries.