Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An improved label for amphetamine fluoroimmunoassay.

G Gallacher1, M Hansell, J Landon

  • 1Department of Chemical Pathology, St. Bartholomews Hospital, London, England.

Therapeutic Drug Monitoring
|September 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mycobacteroides abscessus outbreak and mitigation in a cardiothoracic transplant population: the problem with tap water.

The Journal of hospital infection·2024
Same author

Adrenocorticotrophin.

Journal of the Royal College of Physicians of London·2019
Same author

Occupational health: A positive and proactive approach.

British dental journal·2016
Same author

Complementary surface charge for enhanced capacitive deionization.

Water research·2016
Same author

The role of the clinical biochemist in detection of zinc-induced copper deficiency.

Annals of clinical biochemistry·2015
Same author

Production and assessment of ovine antisera for the manufacture of a veterinary adder antivenom.

The Veterinary record·2014

Researchers synthesized new fluorescein-labeled amphetamine derivatives for immunoassays. This improved assay sensitivity by fourfold, enhancing amphetamine detection without compromising specificity.

Area of Science:

  • Medicinal Chemistry
  • Biochemistry
  • Analytical Chemistry

Background:

  • Immunoassays are crucial for detecting amphetamine.
  • Previous amphetamine immunoassays utilized a specific fluorescein label derived from industrial sources, limiting its general availability.
  • The original assay showed high specificity but could be improved in sensitivity.

Purpose of the Study:

  • To synthesize novel amphetamine derivatives for creating a new fluorescein label.
  • To replace the previously used, industrially sourced label in a fluoroimmunoassay for amphetamine.
  • To evaluate the performance of the new label in terms of specificity and sensitivity.

Main Methods:

  • Synthesis of amphetamine derivatives functionalized at the para-position of the phenyl ring.

Related Experiment Videos

  • Preparation of a fluorescein-labelled amphetamine using the synthesized derivatives.
  • Implementation of the new label in a previously established polarisation fluoroimmunoassay for amphetamine.

    • Main Results:

    • Successful synthesis of novel amphetamine derivatives.
    • Development of a new fluorescein-labelled amphetamine suitable for immunoassay use.
    • The new label maintained similar specificity to the original label.
    • Assay sensitivity was improved by a factor of four with the new label.
    • The enhanced sensitivity is attributed to the elimination of bridge-binding effects.

    Conclusions:

    • Novel amphetamine derivatives can be synthesized and used to create effective fluorescent labels.
    • The new fluorescein-labelled amphetamine offers improved sensitivity in fluoroimmunoassays.
    • Elimination of bridge-binding effects is key to enhancing assay sensitivity.
    • This work provides a more accessible and sensitive method for amphetamine detection via fluoroimmunoassay.