Tissue Dissociation for Metastasis Studies
Related Experiment Video
Updated: Feb 13, 2026

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue
Published on: October 21, 2021
Farhia Kabeer1, Katrina Podsypanina2
1Institut de Recherches Cliniques de Montréal, Quebec H2W 1R7, Canada; Department of Medicine, Experimental Medicine Division, McGill University, Montréal, Quebec H3A 13A, Canada.
Abstract:
The main requirement for most metastasis-related applications is the conversion of solid tissue into a single-cell suspension. In theory, this suspension represents the diversity of cells present in the tissue, whether malignant or benign. We have found that cell viability, as measured by trypan blue staining or fluorescence-activated cell sorting (FACS), is critical for evaluating the success of the tissue-dissociation procedure. The recommended goal is at least 70% cell viability.
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