Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

18.3K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
18.3K
Transposons01:24

Transposons

2.9K
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
2.9K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

20.5K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
20.5K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

14.0K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
14.0K
LTR Retrotransposons03:08

LTR Retrotransposons

20.4K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
20.4K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.3K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SMURF: soft-segmentation for single-cell reconstruction and topological analysis of spatial transcriptomic data.

Nature communications·2026
Same author

Preoperative Lung Function Assessment and the Impact of Spirometry Misdiagnosis on Mortality Following Cardiac Surgery.

Journal of cardiothoracic and vascular anesthesia·2026
Same author

High-avidity TCR signaling induces a distinct KLR-positive exhaustion state in human tumor-infiltrating CD8 T cells associated with immunotherapy response.

bioRxiv : the preprint server for biology·2026
Same author

Prehabilitation Before Cardiac Surgery and Structural Heart Interventions: An Umbrella Review of Pooled Evidence.

Journal of clinical medicine·2026
Same author

NF-κB and STAT3 signaling uniquely stratify survival in female glioblastoma patients.

iScience·2026
Same author

UBIQUITOUS FUNCTIONAL SYNERGY PARTIALLY EXPLAINS WHY MOST TRANSCRIPTION FACTOR BINDING IS NON-FUNCTIONAL.

bioRxiv : the preprint server for biology·2026
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: Mar 26, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.5K

Transposon Calling Cards.

David Mayhew1, Robi D Mitra1

  • 1Department of Genetics, Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, Missouri 63110.

Cold Spring Harbor Protocols
|February 3, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method using transposon "calling cards" to identify where transcription factors bind in yeast genomes. This technique simplifies studying gene regulation networks by tagging DNA binding sites.

More Related Videos

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
10:34

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

Published on: February 1, 2013

14.8K
Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
11:36

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

Published on: September 23, 2017

16.9K

Related Experiment Videos

Last Updated: Mar 26, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.5K
Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
10:34

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

Published on: February 1, 2013

14.8K
Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
11:36

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

Published on: September 23, 2017

16.9K

Area of Science:

  • Molecular Biology
  • Genomics
  • Yeast Genetics

Background:

  • Understanding gene transcription regulation is crucial for deciphering cellular functions.
  • Identifying transcription factor binding sites is a key challenge in genomics.
  • Current methods for mapping transcription factor targets can be labor-intensive.

Purpose of the Study:

  • To develop a novel, efficient method for identifying genomic targets of transcription factors in yeast.
  • To facilitate the study of large numbers of transcription factors and their regulatory roles.
  • To reduce the labor associated with mapping transcription factor binding sites.

Main Methods:

  • Developed a technique using transposon "calling cards".
  • Transcription factors direct the Ty5 retrotransposase to insert barcoded transposons near binding sites.
  • The method is designed for multiplexing with numerous barcoded transcription factors.

Main Results:

  • Successfully demonstrated a method to tag transcription factor binding sites in the yeast genome.
  • The transposon insertion strategy links binding events to specific barcoded transcription factors.
  • The approach has the potential to significantly decrease the labor required for large-scale transcription factor studies.

Conclusions:

  • The transposon "calling card" method offers a powerful and potentially more efficient approach to map transcription factor genomic targets in yeast.
  • This technique can accelerate the understanding of gene regulatory networks by enabling high-throughput analysis.
  • Further application of this method could revolutionize the study of transcription factor function in various organisms.