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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.7K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Jan 18, 2026

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
09:56

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

523

Editing the Mouse Genome Using the CRISPR-Cas9 System.

Adam Williams1, Jorge Henao-Mejia2, Richard A Flavell3

  • 1The Jackson Laboratory for Genomic Medicine, Department of Genetics and Genome Sciences, University of Connecticut Health Center, Farmington, Connecticut 06032;

Cold Spring Harbor Protocols
|February 3, 2016
PubMed
Summary
This summary is machine-generated.

The CRISPR-Cas9 system offers a simpler, faster, and more affordable method for precise genome engineering in mice. This advanced technology enables researchers to rapidly develop in vivo mouse models for complex biological questions.

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Last Updated: Jan 18, 2026

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Genomic engineering in mice is crucial for biological research.
  • Traditional methods are expensive and inefficient.
  • Previous technologies like zinc finger nucleases and TALENs improved precision but remained complex and costly.

Purpose of the Study:

  • To introduce the CRISPR-Cas9 system as a revolutionary tool for mouse genome engineering.
  • To highlight the practical advantages of CRISPR-Cas9 over existing technologies.
  • To discuss considerations for implementing Cas9 in mouse genome editing.

Main Methods:

  • Utilizes the CRISPR-Cas9 system for targeted DNA modification.
  • Focuses on the design and generation of CRISPR-Cas9 reagents.
  • Emphasizes the development of in vivo mouse models.

Main Results:

  • CRISPR-Cas9 provides highly efficient and precise genome editing.
  • Reagents are simple to design and inexpensive to generate.
  • Enables transition from hypothesis to in vivo mouse model in under a month.

Conclusions:

  • The CRISPR-Cas9 system democratizes genome engineering by reducing cost and complexity.
  • Its speed and efficiency allow for the investigation of previously inaccessible research questions.
  • Practical considerations for using Cas9 in mouse genome engineering are discussed.