Related Experiment Video
Updated: Mar 26, 2026
Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies
Published on: January 3, 2011
[Confocal microscopy for the diagnostics of fungal keratitis]
L Daas1, A Viestenz2, M Bischoff2
1Klinik für Augenheilkunde, Universitätsklinikum des Saarlandes UKS, Kirrberger Straße 100, Gebäude 22, 66424, Homburg/Saar, Deutschland. loay.daas@uks.eu.
Abstract:
Fungal keratitis is a rare but very serious eye disease in industrial nations with a frequency of 1-5 % of all forms of keratitis from microbial causes. We present two patients with keratitis of primary unknown cause. Using confocal microscopy fungal filaments could be identified that partially showed a parallel configuration (like "railway tracks"). Thus, the correct diagnosis can often be made and suitable therapy can be non-invasively initiated even before the results of in vitro cultivation (fungal culture), polymerase chain reaction (PCR) and histological investigations are available.
Insights
Fungal keratitis, a serious eye infection, can be diagnosed non-invasively. Confocal microscopy reveals fungal filaments, enabling prompt treatment before lab results are back.
Area of Science:
- Ophthalmology
- Medical Mycology
Background:
- Fungal keratitis is a rare but severe ocular infection, accounting for 1-5% of microbial keratitis cases in industrialized nations.
- Early diagnosis and treatment are crucial for preventing vision loss.
Observation:
- Two patients presented with keratitis of unknown etiology.
- Confocal microscopy revealed fungal filaments with a characteristic parallel configuration, resembling "railway tracks".
Findings:
- Confocal microscopy allows for rapid, non-invasive diagnosis of fungal keratitis.
- Identification of fungal filaments via microscopy enables timely therapeutic interventions.
Implications:
- This diagnostic approach can expedite treatment initiation for fungal keratitis.
- Confocal microscopy offers a valuable tool for managing ocular fungal infections, potentially improving patient outcomes.
Related Concept Videos
01:16Confocal Fluorescence Microscopy
01:28Three-Dimensional Microscopy in Microbiology