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[Simultaneous determination of 54 elements in human whole blood by inductively coupled plasma mass spectrometry]
Qinlong Zhang1, Ge Gao, Yapan Liu
1Chengdu Centre for Disease Control and Prevention, Chengdu 610041, China.
Objective:
To establish a method for simultaneous determination of 54 elements in whole blood by inductively coupled plasma mass spectrometry (ICP-MS).
Methods:
The whole blood sample was digested with nitric acid and hydrogen peroxide in a water bath at 90°C, and then analyzed by ICP-MS with 0.1% ethanol as an matrix-matching agent.
Results:
A good linear relationship was achieved when the concentrations of the 54 elements in whole blood were in the standard range (all r >0.999). The recovery rate of the sample plus the standard was between 80% and 106%, and the relative standard deviation was less than 5%. The standard material of whole blood was determined and the results met the certification requirements.
Conclusion:
The method is simple, rapid, sensitive, and accurate. It is applicable for simultaneous determination of multi-elements in a large number of whole blood samples.
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