Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

24.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.6K
MicroRNAs01:22

MicroRNAs

12.0K
12.0K
The Tumor Microenvironment02:17

The Tumor Microenvironment

8.1K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
8.1K
The Tumor Microenvironment02:17

The Tumor Microenvironment

3.0K
3.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

5.1K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Π-Π stacking stabilized polymeric micelles for hydrophobic drug delivery in the treatment of leishmaniasis.

International journal of pharmaceutics·2025
Same author

Targeted inhibition of hepatic de novo ceramide synthesis ameliorates MASH.

Science advances·2025
Same author

An Immunomodulating Regenerating Hydrogel That Rescues the Oxidative Microenvironment and Reverses Cell Senescence for Osteoporotic Bone Defects.

ACS nano·2025
Same author

Desmoplastic tumor priming using clinical-stage corticosteroid liposomes.

Cell biomaterials·2025
Same author

Hydrophobic ion pairing enables co-loading of water-soluble drugs in polymeric micelles.

Journal of controlled release : official journal of the Controlled Release Society·2025
Same author

Multidrug micelles and sonopermeation for chemotherapy co-delivery to brain tumors.

Journal of controlled release : official journal of the Controlled Release Society·2025

Related Experiment Video

Updated: Mar 26, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

9.0K

MicroRNA Targeting to Modulate Tumor Microenvironment.

Praneeth R Kuninty1, Jonas Schnittert1, Gert Storm2

  • 1Targeted Therapeutics Section, Department of Biomaterials, Science and Technology, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente , Enschede , Netherlands.

Frontiers in Oncology
|February 3, 2016
PubMed
Summary

MicroRNAs (miRNAs) regulate intercellular communication within the tumor microenvironment, influencing cancer progression. This review explores dysregulated miRNAs in stromal cells and potential non-viral delivery systems for miRNA therapeutics.

Keywords:
cancer-associated fibroblastsgene deliverymicroRNAtumor microenvironmenttumor stromatumor-associated macrophages

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

493

Related Experiment Videos

Last Updated: Mar 26, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

9.0K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

493

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Stromal cells and tumor cells communicate, driving cancer growth, angiogenesis, invasion, and metastasis.
  • MicroRNAs (miRNAs) are key regulators of cellular processes and are implicated in cancer development and progression.
  • Dysregulation of miRNAs in the tumor microenvironment significantly impacts intercellular crosstalk.

Purpose of the Study:

  • To provide a comprehensive overview of miRNAs dysregulated in various stromal cells.
  • To elucidate the impact of these miRNAs on intercellular crosstalk within the tumor microenvironment.
  • To discuss the therapeutic potential of miRNAs for modulating the tumor microenvironment and review non-viral delivery systems.

Main Methods:

  • Literature review of studies on miRNAs in the tumor microenvironment.
  • Analysis of miRNA dysregulation in cancer-associated fibroblasts, tumor-associated macrophages, pericytes, endothelial cells, and immune cells.
  • Review of non-viral miRNA delivery systems for targeting stromal cells.

Main Results:

  • miRNAs are significantly dysregulated in diverse stromal cell populations within the tumor microenvironment.
  • These dysregulated miRNAs play crucial roles in regulating intercellular communication and promoting cancer progression.
  • Various non-viral delivery systems show promise for therapeutic miRNA targeting of stromal cells.

Conclusions:

  • Targeting dysregulated miRNAs in stromal cells offers a promising therapeutic strategy for cancer treatment.
  • Modulating intercellular crosstalk via miRNA therapeutics can inhibit tumor growth, angiogenesis, invasion, and metastasis.
  • Overcoming miRNA delivery challenges with non-viral systems is critical for clinical translation of miRNA-based cancer therapies.