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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 26, 2026

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Published on: August 26, 2021

Measuring Apoptosis by Microscopy and Flow Cytometry.

Emilie Hollville¹, Seamus J Martin¹

¹Molecular Cell Biology Laboratory, Department of Genetics, Trinity College, Dublin, Ireland.

Current Protocols in Immunology

|February 3, 2016

Summary

This summary is machine-generated.

Apoptosis is programmed cell death crucial for development and tissue balance, involving caspases and distinct morphological changes. This study details methods to measure apoptosis and differentiate it from necrosis.

Keywords:

apoptosis cell morphology flow cytometry microscopy necrosis

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Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

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Published on: August 26, 2021

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Published on: November 17, 2011

Area of Science:

Cell Biology
Biochemistry

Background:

Apoptosis, or programmed cell death, is vital for development and tissue homeostasis.
It is triggered by diverse stimuli and executed by caspases, leading to characteristic cellular changes.
Necrosis is a distinct, non-programmed cell death pathway often caused by severe damage.

Purpose of the Study:

To describe protocols for measuring apoptosis.
To provide methods for distinguishing apoptosis from necrosis.

Main Methods:

Measurement of apoptotic markers.
Comparative analysis of cellular morphology and integrity.
Assessment of caspase activation.

Main Results:

Apoptosis characterized by cell shrinkage, blebbing, chromatin condensation, and DNA fragmentation.
Necrosis involves cell swelling and plasma membrane rupture.
Distinct biochemical and morphological profiles differentiate the two processes.

Conclusions:

Protocols enable accurate measurement and differentiation of apoptosis and necrosis.
Understanding these cell death pathways is critical for various physiological and pathological conditions.