Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Classification and Identification01:28

Methods of Classification and Identification

1.7K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.7K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

831
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
831
DNA Isolation01:24

DNA Isolation

46.2K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
46.2K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

7.2K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatiotemporal analysis and prediction of severe fever with thrombocytopenia syndrome in Qingdao city, China, 2014-2025.

Infectious diseases (London, England)·2026
Same author

A Self-Reinforcing LGR5-Wnt/β-Catenin-Nedd4L Circuit Drives Fibrotic Progression in Diabetic Kidney Disease.

Diabetes·2026
Same author

Rhodiola sacra Protects Against Hippocampal Neuronal Apoptosis in Chronic Cerebral Ischemia via SIRT1-Driven Mitochondrial Biogenesis.

Current neuropharmacology·2026
Same author

Quadruplet daratumumab in combination with bortezomib, lenalidomide, and dexamethasone as front-line regimen improved clinical outcomes in high-risk myeloma patients in China: a multi-centered prospective real-world study.

BMC cancer·2026
Same author

Risk factors for venous thromboembolism in newly diagnosed multiple myeloma patients based on thrombosis prophylaxis.

Annals of hematology·2026
Same author

Mechanistic insights into inflammatory cytokines in adenomyosis‑induced infertility (Review).

International journal of molecular medicine·2026

Related Experiment Video

Updated: Mar 26, 2026

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
08:55

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Published on: November 1, 2024

2.6K

[Identification of antler powder components based on DNA barcoding technology].

Jing Jia, Lin-chun Shi, Zhi-chao Xu

    Yao Xue Xue Bao = Acta Pharmaceutica Sinica
    |February 4, 2016
    PubMed
    Summary

    DNA barcoding effectively authenticated commercial antler powders, revealing 62% were adulterated with non-pharmacopoeia species like reindeer. This technology ensures traditional Chinese medicine quality and safety.

    More Related Videos

    Field Identification of Matricaria chamomilla using a Portable qPCR System
    12:08

    Field Identification of Matricaria chamomilla using a Portable qPCR System

    Published on: October 10, 2020

    7.4K
    A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
    07:40

    A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

    Published on: March 13, 2011

    21.5K

    Related Experiment Videos

    Last Updated: Mar 26, 2026

    Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
    08:55

    Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

    Published on: November 1, 2024

    2.6K
    Field Identification of Matricaria chamomilla using a Portable qPCR System
    12:08

    Field Identification of Matricaria chamomilla using a Portable qPCR System

    Published on: October 10, 2020

    7.4K
    A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
    07:40

    A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

    Published on: March 13, 2011

    21.5K

    Area of Science:

    • Molecular Biology
    • Pharmacology
    • Zoology

    Background:

    • Antler powder is a valuable ingredient in traditional Chinese medicine.
    • Authenticity and species identification of antler powder are crucial for efficacy and safety.
    • Current authentication methods may not be sufficient to detect adulteration.

    Purpose of the Study:

    • To authenticate the species composition of commercial antler powders using DNA barcoding.
    • To identify adulterants and assess the accuracy of product labeling.
    • To establish DNA barcoding as a reliable tool for quality control in traditional Chinese medicine.

    Main Methods:

    • DNA barcoding targeting the Cytochrome c oxidase subunit I (COI) gene.
    • Standard operating procedures (SOP) for DNA barcoding.
    • Cloning method to resolve mixed COI sequences in complex samples.
    • PCR product sequencing of commercial antler powder samples.

    Main Results:

    • 65 COI sequences were successfully obtained from commercial antler powders.
    • Only 38% of samples were derived from the pharmacopoeia-listed species (Cervus nippon, Cervus elaphus).
    • 62% of samples contained adulterants, with Rangifer tarandus being the most frequent. Adulteration was found across various regions, companies, and price points.
    • Cloning method confirmed C. elaphus and C. nippon as main components in some samples, with some products mislabeled.

    Conclusions:

    • DNA barcoding accurately and efficiently identifies the exact content of commercial antler powder.
    • The study highlights significant issues with adulteration in the antler powder market.
    • DNA barcoding offers a novel technique to enhance clinical safety and improve quality control of traditional Chinese medicine.