Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Extracellular matrix, cell skeletons, and embryonic development.

¹Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, MA 02115.

American Journal of Medical Genetics

|September 1, 1989

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nature and cellular origin of connective tissue matrix.

Upsala journal of medical sciences·2010

Same author

Transforming growth factor beta (TGFbeta) signalling in palatal growth, apoptosis and epithelial mesenchymal transformation (EMT).

Archives of oral biology·2004

Same author

Laser capture microdissection (LCM) for analysis of gene expression in specific tissues during embryonic epithelial-mesenchymal transformation.

Developmental dynamics : an official publication of the American Association of Anatomists·2004

Same author

New evidence that nuclear import of endogenous beta-catenin is LEF-1 dependent, while LEF-1 independent import of exogenous beta-catenin leads to nuclear abnormalities.

Cell biology international·2002

Same author

The mechanism of palatal clefting in the Col11a1 mutant mouse.

Archives of oral biology·2001

Same author

Epithelial-mesenchymal transformation is the mechanism for fusion of the craniofacial primordia involved in morphogenesis of the chicken lip.

Developmental biology·2000

Same journal

Abstracts for the Xth World Congress of Psychiatric Genetics. Brussels, Belgium, 9-13 October 2002.

American journal of medical genetics·2003

Same journal

Defects of blastogenesis.

American journal of medical genetics·2002

Same journal

Malformations of the craniofacial region: evolutionary, embryonic, genetic, and clinical perspectives.

American journal of medical genetics·2002

Same journal

Limb anomalies: Developmental and evolutionary aspects.

American journal of medical genetics·2002

Same journal

Molecular etiology of gut malformations and diseases.

American journal of medical genetics·2002

Same journal

Status of the human malformation map: 2002.

American journal of medical genetics·2002

See all related articles

The cell cytoskeleton, including actin and intermediate filaments, mediates extracellular matrix (ECM) effects on cell shape and migration. Vimentin-actin interactions are key for epithelial cells transforming into mesenchymal cells.

Area of Science:

Cell Biology
Developmental Biology
Biophysics

Background:

The extracellular matrix (ECM) influences epithelial cell differentiation and mesenchymal cell migration.
The role of the cell cytoskeleton in mediating ECM effects on cell behavior is not fully understood.

Purpose of the Study:

To investigate how the cell skeleton (actin, microtubules, intermediate filaments) mediates ECM effects on mesenchymal cell morphology, migration, and formation.
To explore the role of vimentin in epithelial-mesenchymal transformation.

Main Methods:

Examined interactions between epithelial and mesenchymal cells with ECM.
Focused on the role of the actin cortex, microtubules, and intermediate filaments.

Main Results:

Related Experiment Videos

The actin cortex is essential for mesenchymal cell shape, elongation, and matrix migration.
A hypothesis suggests the endoplasm moves past an anchored actin cortex-receptor-matrix complex for cell locomotion.
Epithelial-mesenchymal transformation involves a switch from keratin to vimentin intermediate filaments, altering cell-matrix interactions.

Conclusions:

The cell cytoskeleton, particularly the actin cortex, is crucial for mediating ECM's influence on cell behavior.
Vimentin-actin interactions with the ECM are likely critical for cells to acquire a mesenchymal phenotype.