Immunohistochemical dystrophin reaction in synaptic regions

To investigate the functional significance of dystrophin, we studied various tissues of control and mdx mice, and rats immunohistochemically, using anti-dystrophin antibodies. In control animals, we observed the immunohistochemical localization of dystrophin in synaptic regions such as neuromuscular junctions, the cornea and outer plexiform layer of the retina, the taste buds and neurons in the brain, as well as on the surface membrane of skeletal, cardiac and smooth muscle fibers. In mdx mice, dystrophin was absent from the surface membrane of muscle fibers and the outer plexiform layer of the retina. These results suggest that dystrophin plays an important physiological and/or structural role in the conduction system.