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Microfluidic chip-based silver nanoparticles aptasensor for colorimetric detection of thrombin.

Yaju Zhao1, Xiaohui Liu1, Jie Li1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.

Talanta
|February 4, 2016
PubMed
Summary

A novel colorimetric silver nanoparticles aptasensor (aptamer-AgNPs) enables simple protein detection on a microfluidic chip. This aptasensor accurately detects thrombin at low concentrations, offering a sensitive and selective diagnostic tool.

Keywords:
AptamerColorimetric detectionMicrofluidic chipProteinSilver nanoparticles

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Protein detection is crucial for disease diagnosis and monitoring.
  • Existing methods often require complex procedures and specialized equipment.
  • Developing simple, sensitive, and selective biosensors is an ongoing challenge.

Purpose of the Study:

  • To develop a colorimetric silver nanoparticles aptasensor (aptamer-AgNPs) for protein detection.
  • To implement this aptasensor on a microfluidic chip for enhanced efficiency.
  • To demonstrate the aptasensor's capability in detecting thrombin with high sensitivity and selectivity.

Main Methods:

  • Fabrication of surface-functionalized microfluidic channels as a capture platform.
  • Development of aptamer-conjugated silver nanoparticles (aptamer-AgNPs) for target binding.
  • Colorimetric detection of protein-aptamer-AgNPs complexes within the microfluidic chip.
  • Utilizing thrombin as a model target protein for performance evaluation.

Main Results:

  • The aptasensor successfully detected thrombin via a color change proportional to its concentration.
  • Achieved a low detection limit of 20pM for thrombin without signal amplification.
  • Demonstrated good selectivity for thrombin over other proteins.
  • The method requires minimal sample volume and offers straightforward visual or scanned analysis.

Conclusions:

  • The developed aptamer-AgNPs colorimetric assay on a microfluidic chip provides a simple, sensitive, and selective method for protein detection.
  • This platform shows potential for detecting other protein targets with suitable ligands.
  • The technology offers a promising strategy for point-of-care diagnostics and biochemical analysis.