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Updated: Mar 26, 2026
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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
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A population-based study of aerococcal bacteraemia in the MALDI-TOF MS-era
E Senneby1,2, L Göransson3, S Weiber3
1Clinical Microbiology, Lund, Region Skåne, Sweden. erik.senneby@med.lu.se.
Summary
Aerococcal bacteraemia, particularly from Aerococcus urinae, is increasingly identified in the MALDI-TOF MS era. This study details its incidence, clinical features, and antibiotic susceptibility, finding low MICs for penicillins and carbapenems.
Area of Science:
- Clinical Microbiology
- Infectious Diseases
- Bacteriology
Background:
- Aerococcal bacteraemia is an emerging concern.
- Accurate identification and susceptibility data are crucial for effective treatment.
Purpose of the Study:
- Determine the incidence of aerococcal bacteraemia in the MALDI-TOF MS era.
- Describe clinical presentations and antibiotic susceptibility of aerococci.
Main Methods:
- Retrospective analysis of blood cultures (2012-2014).
- Species identification using MALDI-TOF MS, 16S rRNA gene sequencing, and PYR test.
- MIC determination via Etests.
Main Results:
- Identified 77 patients with aerococcal bacteraemia (A. urinae, A. viridans, A. sanguinicola, A. christensenii).
- Incidence rates reported per million inhabitants.
- A. urinae was the most common species, often associated with urinary tract issues and infective endocarditis.
- Most isolates showed low MICs to penicillins and carbapenems.
Conclusions:
- MALDI-TOF MS has improved aerococcal bacteraemia identification.
- A. urinae is the predominant species causing bacteraemia and infective endocarditis.
- Aerococci remain susceptible to penicillins and carbapenems.
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