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Related Experiment Video

Updated: Mar 26, 2026

Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
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Lysozyme loading and release from Se doped hydroxyapatite nanoparticles.

Yanhua Wang1, Hang Hao2, Shengmin Zhang2

  • 1Medical Science College of China Three Gorges University, Yichang, China.

Materials Science & Engineering. C, Materials for Biological Applications
|February 4, 2016
PubMed
Summary
This summary is machine-generated.

Selenium-doped hydroxyapatite (HA) nanocomposites enhance lysozyme (LSM) loading and slow its release. This study reveals how selenium concentration impacts protein structure and delivery from HA nanoparticles for bone repair applications.

Keywords:
HydroxyapatiteLysozymeNanoparticleProtein releaseSelenium

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Drug Delivery

Background:

  • Element-substituted hydroxyapatite (HA) nanocomposites are promising for bone defect repair.
  • Selenium-substituted HA nanoparticles show potential for bone tumor therapy and osteointegration.
  • The interaction between selenite ions, HA nanoparticles, and protein drugs requires further investigation.

Purpose of the Study:

  • To investigate the influence of selenium doping concentration on lysozyme (LSM) loading and release from HA nanoparticles.
  • To characterize selenium-substituted HA-LSM composites.
  • To analyze the effect of selenium on LSM structure and enzymatic activity during release.

Main Methods:

  • Synthesis and characterization of selenium-substituted HA-LSM composites with varying Se:P ratios.
  • Lysozyme loading efficiency assessment.
  • In vitro lysozyme release studies in phosphate buffer solution (PBS).
  • Analysis of protein structural changes using spectroscopic methods.
  • Evaluation of released lysozyme's enzymatic activity.

Main Results:

  • Composites with Se:P=10% exhibited the highest lysozyme loading (41.7%), compared to undoped HA (34.1%).
  • Selenium doping altered LSM structure, increasing β-sheet and unordered content while decreasing α-helix, β-turns, and self-association.
  • Selenium addition significantly slowed lysozyme release, with Se:P=10% showing a slightly slower rate than other doped samples.
  • Released lysozyme largely retained its enzymatic activity.

Conclusions:

  • Selenium substitution in HA nanoparticles modulates protein drug loading, structure, and release kinetics.
  • Optimized selenium doping (Se:P=10%) enhances lysozyme loading and provides sustained release while preserving bioactivity.
  • These findings support the potential of selenium-substituted HA nanocomposites as advanced drug delivery systems for bone regeneration.