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NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force
Chenyu Li1, Chenyi Xue2, Qiaoyun Yang1
1Department of Biological Sciences, Faculty of Science, National University of Singapore, 14 Science Drive 4, Singapore 117543, Republic of Singapore.
Nature Communications
|February 4, 2016
Summary
Nuclear spindle-associated protein (NuSAP) is crucial for chromosome oscillation during cell division. This study reveals NuSAP regulates the chromokinesin Kid, impacting chromosome movement and alignment.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Chromosome alignment during metaphase is vital for accurate cell division in vertebrates.
- Microtubule-associated proteins, such as NuSAP, are essential for stabilizing spindle microtubules.
- The precise mechanisms regulating chromosome oscillation remain incompletely understood.
Purpose of the Study:
- To investigate the role of NuSAP in chromosome oscillation during metaphase.
- To identify novel regulators of chromosome movement and alignment.
- To elucidate the interplay between NuSAP and the chromokinesin Kid.
Main Methods:
- 3D time-lapse live-cell imaging was employed to observe chromosome dynamics.
- NuSAP and Kid depletion were performed in various cellular configurations (monopolar, bipolar).
- Kinetochore microtubule dynamics were manipulated to analyze force generation.
Main Results:
- Depletion of NuSAP significantly reduced the amplitude and velocity of chromosome oscillation.
- NuSAP was identified as a novel regulator of the chromokinesin Kid.
- Analysis revealed NuSAP's contribution to polar forces at kinetochore microtubules and Kid-mediated polar ejection forces.
Conclusions:
- NuSAP plays a pivotal role in regulating chromosome oscillation during metaphase.
- NuSAP influences chromosome alignment by modulating kinetochore and Kid-dependent forces.
- This study uncovers a new regulatory pathway for chromosome segregation.
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