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Related Experiment Video

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Transcriptome Analysis of Murine Olfactory Sensory Neurons during Development Using Single Cell RNA-Seq.

Paul Scholz1, Benjamin Kalbe1, Fabian Jansen1

  • 1Department of Cell Physiology, Ruhr-University Bochum, 44801 Bochum, NRW, Germany.

Chemical Senses
|February 4, 2016
PubMed
Summary

Researchers analyzed the gene expression of individual olfactory sensory neurons (OSNs) in mice. They discovered that while adult OSNs predominantly express one olfactory receptor (OR), younger mice show varied OR expression patterns.

Keywords:
developmentolfactionolfactory receptorssingle celltranscriptome analysis

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Area of Science:

  • Neuroscience
  • Genomics
  • Molecular Biology

Background:

  • Mammalian olfaction relies on olfactory sensory neurons (OSNs) for survival.
  • The gene expression and regulatory profiles of individual OSNs remain largely uncharacterized.

Purpose of the Study:

  • To investigate the transcriptomic profiles of individual OSNs at different developmental stages.
  • To identify the repertoire of olfactory receptors (ORs) expressed by OSNs.

Main Methods:

  • Isolation of individual GFP-labeled OSNs from Olfr73-GFP mice.
  • Next Generation Sequencing (NGS) for detailed transcriptome analysis.

Main Results:

  • Characterized the olfactory receptor (OR) repertoire in individual OSNs.
  • Identified Olfr73 as a predominant OR in adult OSNs, with 20 additional ORs detected at lower levels.
  • Observed unstable OR patterns in OSNs from earlier developmental stages, with more than one predominant OR per OSN.

Conclusions:

  • The study provides the first detailed transcriptome analysis of individual mammalian OSNs.
  • Findings reveal developmental changes in OR expression patterns within OSNs.
  • Highlights the complexity of olfactory receptor expression in mammalian olfaction.