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Updated: Mar 26, 2026

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
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A Structure-Guided Switch in the Regioselectivity of a Tryptophan Halogenase

Sarah A Shepherd1, Binuraj R K Menon1, Heidi Fisk1

  • 1School of Chemistry and Manchester Institute of Biotechnology, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.

Chembiochem : a European Journal of Chemical Biology
|February 4, 2016
PubMed
Abstract

Keywords:
aryl halidesbiocatalysishalogenationmutagenesisregioselectivity

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