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Related Concept Videos

IR Spectrometers01:25

IR Spectrometers

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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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IR Frequency Region: Fingerprint Region01:03

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IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
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High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
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Introducing Discrete Frequency Infrared Technology for High-Throughput Biofluid Screening.

Caryn Hughes1,2, Graeme Clemens2, Benjamin Bird3

  • 1University of Manchester, School of Chemical Engineering and Analytical Science, Manchester, M13 9PL, United Kingdom.

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Rapid infrared imaging of dried biofluids enables fast and accurate cancer diagnostics. This high-throughput molecular analysis technique shows potential for early detection using liquid biopsies.

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Area of Science:

  • Biomedical optics
  • Molecular spectroscopy
  • Diagnostic technology

Background:

  • Early diagnosis significantly impacts patient survival and quality of life.
  • Biofluids are crucial for early diagnosis due to accessibility and biological relevance.
  • Mid-infrared (mid-IR) imaging of dried biofluid deposits offers a high-throughput molecular analysis method.

Purpose of the Study:

  • To develop and validate a rapid, reproducible mid-IR imaging technique for molecular analysis of dried biofluids.
  • To demonstrate the potential of this method for rapid cancer diagnostics using liquid biopsies.

Main Methods:

  • Utilized tuneable quantum cascade lasers for discrete frequency infrared data collection.
  • Employed targeted frequency scanning to optimize spectral quality, reproducibility, and reduce acquisition time.
  • Analyzed dried serum spots, measuring relative standard deviation (RSD) at different frequency counts (199, 14, and 9 discrete frequencies).

Main Results:

  • Achieved high reproducibility with RSDs of 0.6%, 5.1%, and 15% for 199, 14, and 9 discrete frequencies, respectively.
  • Successfully classified 40 unique dried liquid biopsies from brain, breast, lung, and skin cancer patients against 10 non-cancer controls.
  • Achieved classification accuracies of up to 90% with a rapid 2.4 cumulative seconds acquisition time.

Conclusions:

  • The developed mid-IR imaging methodology is reproducible and suitable for high-throughput molecular analysis.
  • This technique offers a proof-of-concept for rapid, accurate diagnostics of various cancers using liquid biopsies.
  • The method holds significant promise for improving early cancer detection and patient management.