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True histiocytic lymphoma: histopathologic, immunophenotypic and genotypic analysis
C A Hanson1, W Jaszcz, J H Kersey
1Department of Laboratory Medicine and Pathology, University of Minnesota Hospital and Clinic, Minneapolis 55455.
British Journal of Haematology
|October 1, 1989
Summary
True histiocytic lymphoma (THL) exhibits diverse morphology but consistent immunophenotype. Gene rearrangement studies are crucial alongside immunophenotyping and morphology for accurate diagnosis.
Area of Science:
- Hematology
- Oncology
- Immunology
Background:
- True histiocytic lymphoma (THL) is a rare hematologic malignancy.
- Understanding the immunophenotypic and genotypic characteristics of THL is essential for accurate diagnosis and classification.
Purpose of the Study:
- To analyze the immunophenotypic and genotypic profiles of five cases of true histiocytic lymphoma.
- To determine the utility of various diagnostic markers in identifying THL.
Main Methods:
- Immunophenotyping using a panel of histiocytic and lymphoid markers.
- Immunoglobulin and T-cell receptor gene rearrangement studies.
- Morphological and cytochemical analysis.
Main Results:
- Cases displayed morphologic diversity but immunophenotypic homogeneity.
- Malignant cells expressed multiple histiocytic markers (CD11c, CD14, CD68) and HLA-DR.
- Gene rearrangements were detected in three cases, while two retained germline configuration.
Conclusions:
- Certain histiocyte/reticulum cell populations can rearrange immunoglobulin or T-cell receptor genes.
- Accurate THL diagnosis requires a combination of multiple histiocytic markers, cytochemistry, and gene rearrangement studies.
- Interpretation of gene rearrangement must be integrated with immunophenotyping and morphology for definitive cell lineage determination.