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Updated: Mar 26, 2026

Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
Evaluation of MALDI Biotyper Mycobacteria Library v3.0 for Identification of Nontuberculous Mycobacteria
Belén Rodríguez-Sánchez1, M Jesús Ruiz-Serrano2, Adrián Ruiz3
1Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain CIBER de Enfermedades Respiratorias (CIBERES CB06/06/0058), Madrid, Spain Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain mbelen.rodriguez@iisgm.com.
Abstract:
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has demonstrated its ability to promptly identify nontuberculous mycobacteria using the Mycobacteria Library v2.0. However, some species are particularly difficult to identify reliably using this database, providing a low log(score). In this study, the identification power of an updated Mycobacteria Library (v3.0) has been evaluated. Overall, 109 NTM isolates were analyzed with both databases. The v3.0 database allowed a high-level confidence in the identification [log(score) value, ≥1.8] of 91.7% of the isolates versus 83.5% with the v2.0 version (P< 0.01).
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