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Related Experiment Video

Updated: Mar 26, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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Published on: September 24, 2015

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Digital PCR: Principles and Applications.

Rashmi Kanagal-Shamanna1

  • 1Department of Hematopathology, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd. Unit 72, Houston, TX, 77030, USA. Rkanagal@mdanderson.org.

Methods in Molecular Biology (Clifton, N.J.)
|February 5, 2016
PubMed
Summary
This summary is machine-generated.

Digital PCR offers precise absolute quantitation of target molecules. This robust technique, utilizing emulsion or microfluidics, has diverse clinical applications, including BCR-ABL1 transcript analysis.

Keywords:
BCR-ABL1 transcriptDroplet digital PCREmulsion PCRMicrofluidics

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Clinical Diagnostics

Background:

  • Digital PCR (dPCR) is an advanced Polymerase Chain Reaction (PCR) method.
  • It enables highly accurate absolute quantitation of nucleic acid targets.
  • dPCR achieves this by partitioning samples into numerous individual reaction compartments.

Purpose of the Study:

  • To discuss the advantages and clinical utility of digital PCR.
  • To describe a specific droplet digital RT-PCR protocol for BCR-ABL1 transcript analysis.

Main Methods:

  • Automated sample partitioning using "water-in-oil" emulsion (emulsion-based dPCR).
  • Automated sample partitioning using microfluidic chips (microfluidics-based dPCR).
  • Application of droplet digital RT-PCR for identification and absolute quantitation of BCR-ABL1 transcripts.

Main Results:

  • Digital PCR provides precise and accurate absolute quantitation of target molecules.
  • Both emulsion-based and microfluidics-based dPCR enable automated partitioning.
  • The described droplet digital RT-PCR protocol is effective for BCR-ABL1 transcript analysis.

Conclusions:

  • Digital PCR is a powerful tool for absolute quantitation with broad clinical applications.
  • The technique offers advantages over traditional PCR methods for specific targets.
  • Droplet digital RT-PCR is a valuable method for clinical molecular diagnostics, exemplified by BCR-ABL1 quantitation.