Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Postsynaptic Potential (PSP)01:32

Postsynaptic Potential (PSP)

9.6K
Postsynaptic potential (PSP) refers to a change in the electrical potential of a neuron when neurotransmitters released by presynaptic neurons bind to postsynaptic receptors. This potential can either be excitatory, leading to depolarization and ultimately action potential generation, or inhibitory, leading to hyperpolarization and suppression of the postsynaptic neuron.
There are two types of receptors: ionotropic and metabotropic.
The ionotropic receptor is the membrane protein that has an...
9.6K
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

8.2K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
8.2K
Long-term Potentiation01:35

Long-term Potentiation

59.4K
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre- and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
59.4K
Long-term Potentiation01:25

Long-term Potentiation

3.8K
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when...
3.8K
Catenins01:23

Catenins

3.2K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
3.2K
Chemical Synapses01:26

Chemical Synapses

8.2K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of Phelan McDermid Syndrome Estimated To Be ~1:7300 Using a Multisource Model.

Autism research : official journal of the International Society for Autism Research·2026
Same author

Co-expression-based models improve eQTL predictions for transcriptome-wide association studies and highlight new schizophrenia-associated genes.

Nature genetics·2026
Same author

Convergent molecular signatures across eating disorders and obsessive-compulsive disorder in the human brain.

Cell reports·2026
Same author

Altered neurodevelopmental trajectories of brain structure in Tourette syndrome and Chronic Tic Disorders.

medRxiv : the preprint server for health sciences·2026
Same author

Identification of moderate effect size genes in autism spectrum disorder through a novel gene pairing approach.

Communications biology·2026
Same author

Modeling rare coding variation on chromosome X provides insight into the genetics and differential sex prevalence of autism spectrum disorder.

medRxiv : the preprint server for health sciences·2026
Same journal

Vestibular function drives gaze stability in locomoting macaques.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same journal

Region- and layer-specific glutamatergic synapse development in the nascent cortical hierarchy.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same journal

Endogenous peptide derived from c-Cbl-associated protein counteracts its inhibitory effect on enteric neural crest cell colonization in Hirschsprung disease.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same journal

Drowsiness alters the neural dynamics but not the core computations of multisensory integration.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same journal

A Matter of Parameters: Tailored Transcranial Focused Ultrasound Enhances Cortico-Thalamo-Cortical Circuit Resonance.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same journal

Proactive visual and motor prioritization differentially scale with cue reliability.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
See all related articles

Related Experiment Video

Updated: Mar 26, 2026

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
08:13

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

Published on: March 11, 2013

11.4K

Cyfip1 Regulates Presynaptic Activity during Development.

Kuangfu Hsiao1, Hala Harony-Nicolas2, Joseph D Buxbaum3

  • 1Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine and Graduate School of Biomedical Sciences at Mount Sinai, New York, New York 10029.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|February 5, 2016
PubMed
Summary
This summary is machine-generated.

Reduced Cyfip1 levels in juvenile mice lead to larger synapses and faster neurotransmitter release, impacting neural network development and potentially contributing to brain disorders like autism and schizophrenia.

Keywords:
15q11.2F-actinSra1WAVE1WRCsynaptogenesis

More Related Videos

Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture
10:17

Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

Published on: August 2, 2019

8.7K
Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
08:06

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Published on: September 3, 2014

32.2K

Related Experiment Videos

Last Updated: Mar 26, 2026

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
08:13

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

Published on: March 11, 2013

11.4K
Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture
10:17

Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

Published on: August 2, 2019

8.7K
Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
08:06

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Published on: September 3, 2014

32.2K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Gene copy number variations of Cyfip1 are linked to human diseases such as autism and schizophrenia.
  • The precise role of Cyfip1 in brain development and synaptic function remains poorly understood.

Purpose of the Study:

  • To investigate the impact of reduced Cyfip1 levels on the function of developing synapses.
  • To elucidate the molecular mechanisms underlying Cyfip1's role in synaptic development.

Main Methods:

  • Utilized juvenile mice hemizygous for Cyfip1.
  • Employed short hairpin RNA (shRNA)-mediated knockdown and mutant Cyfip1 protein expression.
  • Investigated presynaptic function, protein translation, and F-actin levels.
  • Assessed synaptic responses in hippocampal slices and cultured neurons.
  • Examined the role of Rac1 signaling pathway.

Main Results:

  • Reduced Cyfip1 levels resulted in altered presynaptic function, including decreased paired pulse facilitation and increased miniature EPSC frequency.
  • Synapses with lower Cyfip1 levels exhibited increased size and enhanced vesicle release probability.
  • These alterations were linked to dysregulation of the WAVE regulatory complex and actin polymerization.
  • Inhibition of Rac1 signaling rescued presynaptic responses.

Conclusions:

  • Cyfip1 plays a critical early role in regulating presynaptic activity and synapse formation during neural development.
  • Dysregulation of Cyfip1 impacts synaptic function and may contribute to abnormal neural network formation and maladaptive behaviors.
  • Understanding Cyfip1's function provides insights into the molecular basis of developmental brain disorders.