Comparative analysis of microRNA in schistosomula isolated from non-permissive host and susceptible host

The reed vole Microtus fortis is the only known mammal in which the schistosome is naturally prevented from maturing and schistosome infection does not cause significant pathogenesis. However, the mechanism behind this phenomenon remains unknown. In the present study, Solexa deep sequencing technology was used to carry out high-throughput sequencing and comparative analysis of microRNA (miRNA) between small RNA libraries isolated from 10 days oldschistosomula of M. fortis and BALB/c mice.In total, 10d schistosomula from M. fortis and BALB/c mice yielded 13.37 and 10.84 million reads, respectively, and nearly 39% and 40% of reads could be mapped to selected miRNAs in miRbase. Based on a bioinformatic analysis, we found that most of the miRNAs identified in Schistosoma japonicum were detected in our study. Further analysis revealed that 24 miRNAs were differentially expressed between the schistosomula from the two rodents, of which 21 were down-regulated and three were up-regulated in schistosomula from M. fortis. Also, six novel miRNAs were predicted and identified in this study. Target genes were mapped and filtered by correlating them with differentially expressed genes obtained from S. japonicum oligonucleotide microarray analyses performed in previous studies. miRNAs such as miR-10-3p, miR-10-5p, and miR-2b-5p may affect the growth, differentiation, and metabolism of worms via regulation of the expression of target genes such as enolase, aquaporin, TGF-beta-inducible nuclear protein, and paramyosin. Gene Ontology analysis of the predicted target genes of these six differentially expressed miRNAs revealed that some important biological pathways, such as metabolic processes,glycolysis, and catalytic activity, were involved. The results of this study highlight the function of miRNAs in the development and survival of the schistosome, and provide valuable information to increase our understanding of the regulatory function of miRNAs in schistosome development and host-parasite interactions in a differentially susceptible host environment.