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Evaluation of two methods for direct detection of Fusarium spp. in water
Mariana G Graça1, Inneke M van der Heijden2, Lauro Perdigão1
1Department of Infectious Diseases, Faculty of Medicine, University of São Paulo, Brazil; LIM 54, Institute of Tropical Medicine of the University of São Paulo, Brazil.
Abstract:
Fusarium is a waterborne fungus that causes severe infections especially in patients with prolonged neutropenia. Traditionally, the detection of Fusarium in water is done by culturing which is difficult and time consuming. A faster method is necessary to prevent exposure of susceptible patients to contaminated water. The objective of this study was to develop a molecular technique for direct detection of Fusarium in water. A direct DNA extraction method from water was developed and coupled to a genus-specific PCR, to detect 3 species of Fusarium (verticillioides, oxysporum and solani). The detection limits were 10 cells/L and 1 cell/L for the molecular and culture methods, respectively. To our knowledge, this is the first method developed to detect Fusarium directly from water.
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