Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasma active renin concentration in children.

K Ohyama1, M Ohta, M Fujimoto

  • 1Department of Pediatrics, Yamanashi Medical College, Japan.

Endocrinologia Japonica
|August 1, 1989
PubMed
Summary

Plasma active renin concentration (PARC) is higher in infants and increases with stress or standing. This study validated a reliable method for measuring PARC in children, revealing age-related differences and responses to stimuli.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification and isolation of common and tissue-specific geranylgeranylated gamma subunits of guanine-nucleotide-binding regulatory proteins in various tissues.

European journal of biochemistry·1992
Same author

Extra-adrenal pheochromocytoma-ganglioneuroma. A case report.

Pathology, research and practice·1992
Same author

Developmental regulation of cytochrome P-450 of intrasplenically transplanted fetal hepatocytes in spontaneously hypertensive rats.

Transplantation proceedings·1992
Same author

Manganese-containing superoxide dismutase in blood and urine during open-heart surgery.

Japanese circulation journal·1992
Same author

A Japanese pedigree of autosomal dominant congenital stationary night blindness with variable expressivity.

Ophthalmic paediatrics and genetics·1992
Same author

Effects of daily physical activity on insulin action in the elderly.

Journal of applied physiology (Bethesda, Md. : 1985)·1992

Area of Science:

  • Pediatric Endocrinology
  • Renal Physiology
  • Biochemical Assays

Background:

  • Plasma active renin concentration (PARC) is a key regulator of the renin-angiotensin-aldosterone system.
  • Understanding PARC in children is crucial for diagnosing and managing conditions affecting blood pressure and fluid balance.
  • Existing methods for measuring PARC have limitations, necessitating reliable alternatives.

Purpose of the Study:

  • To validate a renin immunoradiometric assay (IRMA) kit for measuring PARC in children.
  • To compare PARC measurements with plasma renin activity (PRA).
  • To investigate age-related changes and physiological influences on PARC in pediatric populations.

Main Methods:

  • PARC was measured using a renin immunoradiometric assay (IRMA) kit in healthy children aged one month to 16 years.

Related Experiment Videos

  • PARC was compared with plasma renin activity (PRA) in non-diluted plasma samples.
  • PARC levels were analyzed in relation to age, crying state (infants), and body position (upright vs. supine).

    • Main Results:

    • The IRMA method for PARC was found to be simple, reliable, and unaffected by plasma substrate levels or dilution.
    • PARC measurements correlated well with PRA in non-diluted samples.
    • PARC was significantly higher in infants compared to older children, with marked increases during crying.
    • PARC increased significantly in children aged 7-11 years when moving from a supine to an upright position.

    Conclusions:

    • The validated IRMA method provides a reliable tool for assessing PARC in pediatric studies.
    • Infants exhibit higher PARC levels, potentially due to a hyperresponse to acute stress like blood sampling.
    • Short-term postural changes (standing) may stimulate active renin release or activation in normal children.