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Related Experiment Videos

Structure and developmental expression of the chicken CDC2 kinase.

W Krek1, E A Nigg

  • 1Swiss Institute for Experimental Cancer Research (ISREC), Epalinges s/Lausanne.

The EMBO Journal
|October 1, 1989
PubMed
Summary

Chicken cdc2 (cell division cycle 2) gene expression decreases during embryonic development. This cell cycle regulator

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Background:

  • The cdc2 protein kinase is crucial for regulating the eukaryotic cell cycle.
  • Understanding cdc2 gene expression is vital for comprehending cell cycle control during development.

Purpose of the Study:

  • To isolate and characterize the chicken homolog of the cdc2 gene.
  • To investigate the expression patterns of cdc2 mRNA and protein during chicken embryonic development.

Main Methods:

  • Isolation of a chicken cdc2 cDNA clone.
  • Antibody generation against the cdc2 protein.
  • Northern analysis and immunoblotting to study gene and protein expression.
  • Functional analysis by expressing chicken cdc2 in fission yeast.

Main Results:

  • The chicken cdc2 protein shares structural homology with homologs from other species.
  • Chicken cdc2 kinase can functionally rescue a temperature-sensitive cdc2 mutant in yeast, confirming its role as a cell cycle regulator.
  • Both cdc2 mRNA and protein levels significantly decrease in chicken embryos from day 3 to day 11.
  • Minimal cdc2 mRNA or protein is detected in adult chicken tissues (liver, brain, heart, skeletal muscle).

Conclusions:

  • A general correlation exists between cdc2 mRNA abundance and tissue proliferative state.
  • Regulation of cdc2 expression in developing chicken appears to occur at multiple levels, as mRNA and protein levels do not always correlate within individual tissues.

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