Related Experiment Video
Updated: Mar 26, 2026
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
Published on: January 31, 2014
Spherical silver nanoparticles in the detection of thermally denatured collagens
Manuel Ahumada1,2, Sarah McLaughlin1, Natalia L Pacioni3
1Bio-nanomaterials Chemistry and Engineering Laboratory, Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin Street, Rm H5229, Ottawa, Ontario, K1Y 4W7, Canada.
Abstract:
We have developed a rapid colorimetric method to determine the concentration of denatured collagen in solution, which is based on the collagen-silver nanoparticle corona formation. Using the proposed method, the lowest detectable concentration of denatured collagen protein in a solution of pure collagen was 14.7, 8.5, and 8.6 μg mL(-1) for porcine (PCOL), rat tail (RCOL), and type I human recombinant (HCOL) collagen, respectively.
