Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An integrated molecular classification system identifies distinct prognostic clusters of non-muscle-invasive bladder cancer.

Functional & integrative genomics·2026

Same author

EED-Astig: A Multimodal Dataset for Pediatric Astigmatism Severity Prediction.

Scientific data·2026

Same author

Raphe subregion seeds reveal whole-brain functional connectivity differences underlying adolescent depression.

BMC psychiatry·2026

Same author

Pueraria lobata-derived exosome-like nanovesicles alleviate rheumatoid arthritis via targeting <i>Ruminococcus gnavus</i> phenylethylamine production.

Gut·2025

Same author

Transcriptome analysis of differentially expressed genes in lily leaves under selenite application.

Scientific reports·2025

Same author

Ultrafast Dynamics of Porphyrins in the Condensed Phase: II. Zinc Tetraphenylporphyrin<sup>†</sup>.

The journal of physical chemistry. A·2025

Same journal

Costunolide ameliorates autoimmune uveitis by targeting USP15 to suppress TNF-α-induced retinal endothelial inflammation.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

A ligandable PNT domain establishes ERG as a directly targetable oncogenic driver in prostate cancer.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Identification of cellular intermediates unveils unique enzymes for flagellar glycan biosynthesis in <i>Clostridioides difficile</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

The structure of correlated variability reflects task-relevant information in sensory neurons.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Shared neurogenetic substrates of nonplanning impulsivity and procrastination.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

HIV-1 capsid interactions with Nuclear Pore Complex components support nuclear entry via affinity gradient.

Proceedings of the National Academy of Sciences of the United States of America·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 26, 2026

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

Infrared PINEM developed by diffraction in 4D UEM.

Haihua Liu¹, John Spencer Baskin¹, Ahmed H Zewail²

¹Physical Biology Center for Ultrafast Science and Technology, Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, CA 91125.

Proceedings of the National Academy of Sciences of the United States of America

|February 6, 2016

Summary

This summary is machine-generated.

Researchers developed a new method using photon-induced near-field electron microscopy (PINEM) in diffraction space to study infrared light interactions with materials. This advancement offers unprecedented energy resolution for observing ultrafast dynamics at the nanoscale.

Keywords:

diffraction electron microscopy materials science nanostructures ultrafast dynamics

More Related Videos

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

Related Experiment Videos

Last Updated: Mar 26, 2026

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

Area of Science:

Ultrafast electron microscopy
Nanophotonics
Plasmonics

Background:

Four-dimensional ultrafast electron microscopy (4D UEM) observes ultrafast dynamics of photon-matter interactions.
Photon-induced near-field electron microscopy (PINEM) captures photon-electron interactions in nanoplasmonics and nanophotonics.

Purpose of the Study:

To extend PINEM capabilities by utilizing a focused electron beam in diffraction space for infrared light-induced PINEM.
To achieve unprecedented energy resolution for studying ultrafast dynamics in reciprocal and energy space.

Main Methods:

Development of infrared-light-induced PINEM in diffraction space.
Utilizing a focused, nanometer-scale electron beam.
Achieving 0.63 eV energy resolution with a 200-keV electron beam.

Main Results:

First-time resolution of separated PINEM electron-energy spectrum peaks induced by 1,038 nm infrared light.
Similar first-order PINEM peak amplitudes for optical fluences differing by over 60x at a copper-vacuum interface.
Observation and spatial dependence study of the nonlinear regime in IR PINEM under high fluence.

Conclusions:

The developed PINEM diffraction method provides unprecedented energy resolution for ultrafast dynamics studies.
This technique enables detailed investigation of light-matter interactions in reciprocal and energy space with high temporal resolution.
The method opens new avenues for exploring nanoscale phenomena in nanophotonics and plasmonics.