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Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
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Published on: March 22, 2012

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Diagnostics and susceptibility testing in Aspergillus

Leticia Bernal-Martínez1, Ana Alastruey-Izquierdo1, Manuel Cuenca-Estrella1

  • 1Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Madrid, Spain.

Future Microbiology
|February 6, 2016
PubMed
Abstract

Insights

New diagnostic methods for invasive aspergillosis improve early detection in immunocompromised patients. Reviewing novel diagnostics and antifungal resistance is crucial for effective treatment and survival.

Area of Science:

  • Medical Mycology
  • Infectious Diseases
  • Clinical Diagnostics

Background:

  • Invasive aspergillosis poses a significant threat to immunosuppressed individuals, leading to high morbidity and mortality.
  • Conventional diagnostic methods for invasive aspergillosis can be slow, impacting timely treatment initiation.
  • Emerging antifungal resistance in Aspergillus species complicates treatment strategies.

Purpose of the Study:

  • To review novel diagnostic approaches for invasive aspergillosis.
  • To discuss the detection of fungal components like antigens and DNA for early diagnosis.
  • To highlight recent advancements in rapid diagnostic tests and address antifungal resistance.

Main Methods:

  • Review of current literature on diagnostic procedures for invasive aspergillosis.
  • Analysis of newer methods focusing on the detection of fungal antigens and DNA.
  • Examination of intrinsic and acquired antifungal resistance mechanisms, particularly to triazoles in Aspergillus fumigatus.

Main Results:

  • Novel diagnostic procedures, including antigen and DNA detection, offer alternatives to conventional methods.
  • Rapid diagnostic tests show promising results for faster identification of invasive aspergillosis.
  • Antifungal resistance is an increasing concern, necessitating methods for its detection.

Conclusions:

  • Early and accurate diagnosis of invasive aspergillosis is vital for patient survival.
  • Advancements in diagnostic technologies are improving the detection of Aspergillus infections.
  • Monitoring and detecting antifungal resistance are essential for selecting appropriate antifungal agents.
Keywords:
LFDPCRantifungal resistanceazolescryptic speciesdiagnosisgalactomannanβ-glucan

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