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Adenovirus E1A/E1B Transformed Amniotic Fluid Cells Support Human Cytomegalovirus Replication
Natascha Krömmelbein1, Lüder Wiebusch2, Gudrun Schiedner3
1Institute for Virology, University Medical Center of the Johannes Gutenberg-University Mainz, D-55131 Mainz, Germany. kroemme@uni-mainz.de.
Viruses
|February 6, 2016
Summary
A new study shows that adenovirus-immortalized CAP cells support full human cytomegalovirus (HCMV) replication. These cells release infectious HCMV progeny and dense bodies, offering potential for vaccine production.
Area of Science:
- Virology
- Cell Biology
- Vaccine Development
Background:
- Human cytomegalovirus (HCMV) replicates efficiently in fibroblasts but poorly in many other cells.
- Adenovirus-immortalized cell lines have historically resisted HCMV replication.
Purpose of the Study:
- To investigate HCMV replication in a permanent cell line immortalized by adenovirus type 5 E1A and E1B (CAP).
- To assess the potential of CAP cells for vaccine production.
Main Methods:
- Infection of CAP cells with HCMV.
- Analysis of viral protein expression, DNA replication, and progeny production.
- Electron microscopy to detect viral particles and dense bodies.
Main Results:
- CAP cells supported the complete HCMV replication cycle, including viral DNA synthesis and protein expression.
- Infectious HCMV progeny and subviral dense bodies were released from CAP cells, albeit at lower levels than fibroblasts.
- HCMV efficiently entered CAP cells and disrupted PML-bodies.
Conclusions:
- Adenovirus E1A/E1B expression does not universally inhibit HCMV replication.
- CAP cells are a viable substrate for HCMV replication and may be useful for dense body-based vaccine production.
