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Methods for identification of flavobacteria.

M J Pickett1

  • 1Department of Microbiology, University of California, Los Angeles 90024.

Journal of Clinical Microbiology
|October 1, 1989
PubMed
Summary
This summary is machine-generated.

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Detecting Flavobacterium meningosepticum (biovar IIa) and Flavobacterium indologenes (biovar IIb) requires specific methods. Optimal indole detection and starch hydrolysis tests, along with D-arabinose acidification, are key for accurate differentiation.

Area of Science:

  • Clinical Microbiology
  • Bacteriology

Background:

  • Discrepancies exist in literature regarding reliable methods for differentiating Flavobacterium meningosepticum (biovar IIa) and Flavobacterium species CDC group IIb (biovar IIb; Flavobacterium indologenes).
  • These inconsistencies may stem from variations in the diagnostic methodologies employed.

Purpose of the Study:

  • To identify optimal laboratory methods for the accurate detection and differentiation of Flavobacterium meningosepticum (biovar IIa) and Flavobacterium indologenes (biovar IIb).
  • To resolve conflicting reports on key biochemical features used in distinguishing these two bacterial biovars.

Main Methods:

  • Evaluation of indole production using a modified Kovács reagent and buffered tryptophan medium.
  • Comparison of starch hydrolysis capabilities, specifically corn starch versus soluble potato starch.

Related Experiment Videos

  • Assessment of DNA hydrolysis and pigmentation on blood agar plates.
  • Utilization of D-arabinose acidification for definitive biovar differentiation.

    • Main Results:

    • A modified Kovács reagent with buffered tryptophan medium proved optimal for indole detection, though indole production was not universal in either biovar.
    • Hydrolysis of corn starch was a more reliable indicator for distinguishing the biovars than soluble potato starch hydrolysis.
    • Pigmentation was observed in both biovars, but only biovar IIb exhibited obvious pigmentation on 2-day blood agar.
    • Acidification of D-arabinose definitively differentiated the two biovars, with other features offering supplementary but not conclusive evidence.

    Conclusions:

    • Methodological choices significantly impact the reliability of tests used to differentiate Flavobacterium meningosepticum and Flavobacterium indologenes.
    • Acidification of D-arabinose is the most definitive test for distinguishing between biovar IIa and biovar IIb.
    • Standardized protocols employing specific reagents and media are crucial for accurate identification of these clinically relevant Flavobacterium species.