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Jun-Mediated Changes in Cell Adhesion Contribute to Mouse Embryonic Stem Cell Exit from Ground State Pluripotency
Giulia Veluscek1, Yaoyong Li1, Shen-Hsi Yang1
1Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom.
Stem Cells (Dayton, Ohio)
|February 7, 2016
Summary
The transcription factor Jun is crucial for mouse embryonic stem cells (ESCs) to exit pluripotency. Jun promotes cell adhesion, which is essential for timely differentiation.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Molecular Biology
Background:
- Embryonic stem cells (ESCs) possess pluripotency, the ability to differentiate into any cell type.
- Mechanisms regulating ESC differentiation are less understood than those maintaining pluripotency.
- Transcription factors, like Jun, play roles in cell fate decisions.
Purpose of the Study:
- To investigate the role of the transcription factor Jun in mouse ESC differentiation.
- To understand how Jun influences the exit from the pluripotent ground state.
Main Methods:
- Transcriptomic analysis of differentiating mouse ESCs.
- Investigated the impact of Jun modulation on gene expression.
- Assessed the role of Jun-regulated genes in cell adhesion and differentiation.
Main Results:
- Jun is required for upregulating genes involved in cell adhesion during ESC differentiation.
- Several Jun-dependent adhesion genes are essential for efficient cell adhesion.
- This Jun-mediated adhesion is critical for timely exit from pluripotency and early differentiation.
Conclusions:
- Jun plays a key role in promoting ESC differentiation by regulating cell adhesion.
- Adhesion is a critical process for the controlled exit of ESCs from pluripotency.
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