Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preliminary study on PAX1/JAM3 methylation and HPV viral load in CIN3-like squamous cell carcinoma: Are there differences from CIN3 and early invasive carcinoma?

Clinical epigenetics·2026
Same author

Identification of Conserved Cross-Reactive B-Cell Epitopes in CPV1 and CPV2 L1 Proteins with Vaccine Potential.

Vaccines·2026
Same author

Targeted GLP-1 nanotherapy for Wnt/β-catenin activation to enhance endothelial progenitor cell-mediated re-endothelialization and prevent intracranial aneurysm recurrence.

Journal of neurosurgery·2026
Same author

Pinch restoration in post-stroke hemiplegia rehabilitation: A case report of motor task-synchronized precision repetitive transcranial magnetic stimulation.

Brain stimulation·2026
Same author

Targeted Inhibition of MEGF10-Mediated Astrogliosis Reduces Glial Scar Formation and Promotes Neurofunction Recovery in Mice After Stroke.

Glia·2026
Same author

Interferon-γ-Responsive Microglia-Derived Extracellular Vesicles Inhibited Neurogenesis After Stroke via MicroRNA-199a-5p/<i>SIRT1</i> Axis.

Journal of the American Heart Association·2026

Related Experiment Video

Updated: Mar 26, 2026

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
08:22

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

568

The biphasic function of microglia in ischemic stroke.

Yuanyuan Ma1, Jixian Wang2, Yongting Wang3

  • 1Department of Neurology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China; Neuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.

Progress in Neurobiology
|February 7, 2016
PubMed
Summary

Microglia, the brain's immune cells, have a dual role in ischemic stroke, both harming and healing. Therapies should focus on modulating their behavior, not just suppressing activation, for better recovery.

Keywords:
CrosstalkIschemic strokeMicrogliaPolarizationmicroRNAs

More Related Videos

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia
11:36

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Published on: November 14, 2020

10.3K
Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
13:28

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Published on: September 4, 2013

12.0K

Related Experiment Videos

Last Updated: Mar 26, 2026

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
08:22

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

568
A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia
11:36

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Published on: November 14, 2020

10.3K
Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
13:28

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Published on: September 4, 2013

12.0K

Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • Microglia are the primary immune cells of the central nervous system, originating from primitive progenitor cells in the yolk sac.
  • Traditionally, microglia activation post-ischemic stroke was considered detrimental, but emerging evidence highlights their beneficial roles in neuroprotection and recovery.
  • The complex and often opposing functions of microglia suggest a biphasic role, potentially explained by differential polarization.

Purpose of the Study:

  • To comprehensively review the mechanisms regulating microglia activation and polarization in the context of ischemic stroke.
  • To discuss the role of microRNAs in modulating microglia function during brain ischemia.
  • To examine the interactions between microglia and other brain cells (neurons, astrocytes, oligodendrocytes, stem cells) in ischemic stroke.

Main Methods:

  • Literature review and synthesis of existing research on microglia biology and ischemic stroke.
  • Analysis of studies investigating microglia activation, polarization, and their functional outcomes.
  • Examination of molecular mechanisms, including microRNAs, and cell-cell interactions.

Main Results:

  • Microglia activation exhibits a biphasic role in ischemic stroke, contributing to both injury and repair.
  • Differential polarization of microglia is a key factor influencing their pro-inflammatory or anti-inflammatory responses.
  • MicroRNAs and interactions with other brain cells significantly modulate microglia behavior during ischemia.

Conclusions:

  • Future therapeutic strategies for ischemic stroke should aim to modulate microglia polarization rather than solely suppressing activation.
  • Further research is crucial to fully understand the cellular and molecular mechanisms of microglia polarization in the ischemic brain.
  • The roles of microRNAs and stem cells in mediating microglia responses during brain ischemia warrant deeper investigation.