Association between microRNA polymorphisms and chronic pancreatitis
Zhaozhi Wang1, Diangeng Li2, Jianjun Jin1
1Department of Gastroenterology, The First Affiliated Hospital of He'nan University of Science and Technology, Luoyang 471000, China.
Backgroud:
MicroRNAs play important roles in the development and progression of many human diseases. mir-146a could significantly suppress the induction of proinflammatory cytokines IL-1β, IL-6, TNF-α, NF-κB and chemokine MCP-1, which might play important roles in chronic pancreatitis. This study was conducted to evaluate the association between mir-146a rs2910164, a functional polymorphism in the pre-mir-146a, and chronic pancreatitis risk.
Methods:
The rs2910164 genotypes were determined in 165 patients with chronic pancreatitis and 200 healthy controls who were frequency matched for age and gender. One single nucleotide polymorphism (rs2910164) was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RLFP).
Results:
The frequency of individuals who carried [G] allele was significantly higher in cases (62.7%) than in controls (53.7%, p = 0.015), which resulted in a statistically significant pathogenic effect associated with this variant allele (OR: 1.448, CI: 1.076-1.950; p = 0.015). The GC and GG genotypes showed strong and significant increased risk for complication of chronic pancreatitis (OR = 3.668, 95%CI = 1.233-10.916, p = 0.019; OR = 5.667, 95%CI = 1.852-17.336, p = 0.002). The individuals carrying G allele confer a lower expression level of mature mir-146a.
Conclusion:
These findings suggest that the mir-146a rs2910164 may contribute to genetic susceptibility to chronic pancreatitis, and that mir-146a might be involved in chronic pancreatitis development.
Insights
The study found that the mir-146a rs2910164 genetic variant is associated with an increased risk of chronic pancreatitis. Individuals with the G allele have a higher susceptibility to developing this disease.
Area of Science:
- Genetics
- Molecular Biology
- Gastroenterology
Background:
- MicroRNAs are crucial in disease development.
- MicroRNA-146a (miR-146a) can suppress inflammatory cytokines implicated in chronic pancreatitis.
- This study investigates the link between a specific miR-146a polymorphism (rs2910164) and chronic pancreatitis risk.
Purpose of the Study:
- To evaluate the association between the mir-146a rs2910164 polymorphism and chronic pancreatitis.
- To determine if this genetic variant influences susceptibility to chronic pancreatitis.
Main Methods:
- Genotyping of the rs2910164 polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
- Analysis of 165 chronic pancreatitis patients and 200 healthy controls.
- Frequency matching for age and gender in control group.
Main Results:
- The G allele of mir-146a rs2910164 was significantly more frequent in chronic pancreatitis patients (62.7%) compared to controls (53.7%).
- Carriers of the G allele showed a statistically significant increased risk for chronic pancreatitis (OR: 1.448, p=0.015).
- GC and GG genotypes were strongly associated with an increased risk of chronic pancreatitis complications.
Conclusions:
- The mir-146a rs2910164 polymorphism may contribute to genetic susceptibility to chronic pancreatitis.
- These findings suggest a potential role for miR-146a in the pathogenesis of chronic pancreatitis.
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