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Published on: March 21, 2016
Effects of sucrose amendment on ammonia assimilation during sewage sludge composting
Liqiang Meng1, Weiguang Li2, Shumei Zhang3
1School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090 Harbin, China; Institute of Microbiology, Heilongjiang Academy of Sciences, 150010 Harbin, China; Institute of Advanced Technology, Heilongjiang Academy of Sciences, 150020 Harbin, China.
Abstract:
The aim of this study was to evaluate the laboratory-scale composting of sewage sludge and pumice mixtures that were amended with sucrose. The variation in temperature, pH, NH4(+)-N, ammonia emission, bacterial community, ammonia assimilating bacteria (AAB) populations and enzymatic activity related to ammonia assimilation were detected. The addition of sucrose increased the AAB population by 2.5-3.5 times, reduced ammonia emission by 24.7-31.1% compared with the control treatment, and promoted the growth of Bacillus and Wautersiella. The activities of glutamate dehydrogenase (GDH), glutamate synthase (GS) and glutamine synthetase (GOGAT), were enhanced by the addition of sucrose. GDH made a substantial contribution to ammonia assimilation when the ammonia concentration was high (⩾1.5g/kg) in the thermophilic phase. The GS/GOGAT cycle played an important role at low ammonia concentrations (⩽1.1g/kg) in the cooling phase. These results suggested that adding sucrose to sludge compost could promote ammonia assimilation and reduce ammonia emission.
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