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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Published on: May 30, 2017

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Anion inhibition studies of the β-carbonic anhydrase from the pathogenic bacterium Vibrio cholerae

Daniela Vullo1, Sonia Del Prete2, Viviana De Luca3

  • 1Università degli Studi di Firenze, Dipartimento Di Chimica, Laboratorio di Chimica Bioinorganica, Polo Scientifico, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.

Bioorganic & Medicinal Chemistry Letters
|February 9, 2016
PubMed

Insights

Researchers investigated inhibitors for Vibrio cholerae beta-carbonic anhydrase (VchCAβ). Sulfamide and phenylboronic acid were potent inhibitors, offering potential for new antimicrobial drug development.

Area of Science:

  • Biochemistry
  • Microbiology
  • Enzymology

Background:

  • Vibrio cholerae, a pathogen, possesses three carbonic anhydrase (CA) enzymes.
  • Carbonic anhydrases are crucial metalloenzymes involved in various physiological processes.

Purpose of the Study:

  • To investigate the anion inhibition of the beta-class carbonic anhydrase from Vibrio cholerae (VchCAβ).
  • To identify potential selective inhibitors of VchCAβ for pharmacological applications.

Main Methods:

  • Anion inhibition studies were performed on purified VchCAβ.
  • Enzyme kinetics were used to determine inhibition constants (KIs).

Main Results:

  • Sulfamide, sulfamate, phenylboronic acid, and phenylarsonic acid were identified as potent VchCAβ inhibitors (KIs: 54-86 μM).
  • Diethyldithiocarbonate showed moderate inhibition (KI: 0.73 mM).
  • Various other anions exhibited inhibition in the millimolar range.

Conclusions:

  • Selective VchCAβ inhibitors were identified, distinct from human CA isoforms.
  • These inhibitors could serve as valuable tools for studying VchCAβ's physiological roles and for developing novel therapeutics.

Keywords:
AnionsCarbonic anhydraseHydratase activityInhibitorsMetalloenzymesPathogenic bacteriaVibrio cholerae

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